Evaluation of Long-Term Gene Expression in Mouse Liver Using PhiC31 Integrase and Hydrodynamic Injection
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چکیده
منابع مشابه
Impact of Hydrodynamic Injection and phiC31 Integrase on Tumor Latency in a Mouse Model of MYC-Induced Hepatocellular Carcinoma
BACKGROUND Hydrodynamic injection is an effective method for DNA delivery in mouse liver and is being translated to larger animals for possible clinical use. Similarly, phiC31 integrase has proven effective in mediating long-term gene therapy in mice when delivered by hydrodynamic injection and is being considered for clinical gene therapy applications. However, chromosomal aberrations have bee...
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BACKGROUND Achieving long-term gene expression in kidney will be beneficial for gene therapy of renal and congenital diseases, genetic studies constructing animal disease models, and the functional analysis of disease-related genes. PURPOSE The purpose of this study was to develop an in vivo long-term gene expression system in murine kidney using φC31 integrase. METHODS Gene expression in c...
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PURPOSE Gene therapy has shown promise in animal models of retinal disease, with the most success achieved to date with viral vectors used for gene delivery. Viral vectors, however, have side effects and limitations and are difficult to manufacture. The present study was conducted in an attempt to develop a novel system for long-term gene transfer in rat retinal pigment epithelium (RPE), by usi...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 2012
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.b12-00083